We introduce a suitable liquid medium to the test product, transfer it into a special plate, and finally place a Boyden chamber over the same....
Next, we introduce dermal fibroblasts cells into a liquid medium to support their growth & replication. These cells are then placed on top of the filter/porous membrane.
This set-up is left unattended for one day under suitable lab conditions.
Due to the chemotactic (chemical signals to attract cells) property of our test product, the cells travel across the membrane into the test solution....
On day 2, we stain the dermal fibroblasts migrated under the membrane using a suitable dye and track their number through a spectrophotometer.