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Time-Kill Assay
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The time-kill assay is a laboratory method used to determine how well our test product can kill microorganisms over a certain amount of time. The test involves exposing these microorganisms to the test product and tracking their viability at different intervals.
We test our formulations on five challenge microorganisms to check their anti-microbial effect.1
What Are Challenge Test Micro-organsims & Why We Use Them?
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The challenge microorganisms used in the time-kill assay include Candida albicans (yeast - a form of fungus), Aspergillus brasiliensis (filamentous mold - a form of fungus), Escherichia coli (bacterium), Pseudomonas aeruginosa (bacterium), and Staphylococcus aureus (bacterium). They are used in the time-kill assay to test the effectiveness of a test product against a range of pathogenic microorganisms that it may get exposed to. Challenge microorganisms represent a broad spectrum of potential contaminants that could affect product quality or safety.1
How We Test The Antimicrobial Effect Of Our Products?
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Step 1
Introducing The Test Product
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The first step involves preparing a culture of the microorganism of interest. The culture (liquid medium containing the microrganism) is then diluted to a specific concentration to ensure...

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Step 2
Calculating The Initial Pathogenic Population Count
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Next, the initial population count for all five micro-organisms in the test product is calculated by diluting the solution...

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Step 3
Tracking The Growth Of Challenge Micro-organisms
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Similar to the last step, the population count for each challenge micro-organism is calculated by the cell plating method...

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What's The Final Result?
For a product to show antimicrobial properties, the microorganisms should reflect a <99.9% decline in their concentration over the set amount of time. At Areoveda, the tested products reveal similar results!
Pick From Our Range Of Tested Time Kill Assay Products Now
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