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Bone Mineralization
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As the name suggests, a bone mineralization assay is a method to test the ability of an oil or cream-based product to strengthen the process of bone formation. The construction of a bone starts with the laying down of an extra-cellular matrix facilitated by special fibroblast cells known as osteoblasts. Derived from mesenchymal stem cells (MSCs), osteoblasts secrete & form a mineralized bone matrix containing collagen, calcium, phosphorus, etc. that serve as the base for bone development.
We test all our formulations on MG63 fibroblast cell lines to check their bone formation effect.
What Is MG63 Cell Line & Why We Use It?
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MG63 fibroblast cell line has been derived from the bone of a 14-year-old white male suffering from osteosarcoma (bone cancer). These cell lines are an excellent tool for investigating the bone formation effect of our products as they retain and mimic the physiological, biological & developmental properties of osteoblasts in an in vitro (testing in a lab) environment. Moreover, they grow and divide at a much faster rate in comparison to normal osteoblast cell lines.
How We Test The Bone Mineralization Effect Of Our Products?
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Step 1
Osteoblast Differentiation & Matrix Mineralization
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The first step involves supplementing a set of fibroblast cells (MG63) with osteoblast growth media for 7 days. With exposure to essential osteogenic (bone-formation) factors, these cells initiate the process of bone mineralization....

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Step 2
Assessment Of Bone Formation By Alizarin-red Staining
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We track the level of calcium deposition in both sets of cell lines by introducing the Alizarin-red S stain. Alizarin reacts with the calcium cations and produces a bright red color....

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What's The Final Result?
For a product to positively affect the bone formation process, itslevel of calcium deposition should be higher than the untreated control. At Areoveda, the tested products reveal similar results!
Pick From Our Range Of Tested Bone Mineralization Products Now
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