Anti Dandruff Test

Anti-dandruff

Dandruff is a common and persistent inflammatory condition of the scalp characterized by yellowish or flaky white scales. It can also be visible around the nasolabial folds, the area behind our ears, eyebrows, and intertriginous spots of the skin. The most common reason for developing dandruff is attributed to increased levels of a microorganism called Malassezia furfur (fungus) in the scalp region. An anti-dandruff test is a great way to check the ability of our test products to inhibit the growth of this fungal infection.
We test our formulations on Malassezia furfur to check their anti-dandruff effect.

What Is Malassezia furfur & Why We Use Them?

Malassezia furfur constitutes 80% of the total fungal population of the skin microbiota and exists as a commensal yeast. They feed on saturated fatty acids found in sebum & multiply at a fast rate, disturbing the skin renewal process. As a result, these scalp skin cells form clusters that appear as white flakes. Therefore, they serve as an excellent tool for investigating the anti-dandruff effect of our products.

How We Test The Anti-dandruff Property Of Our Products?

Step 1

Bacterial Plating

The first step involves introducing an inoculum (fungal component to initiate infection) of Malassezia furfur into a culture plate with solidified agar gel containing a sufficient supplyof nutrients and vitamins to aid growth & replication. Next, the inoculum is spread evenly on the agarose plate.

Step 2

Calculating The Initial Pathogenic Population Count

Next, the initial population count for Malassezia furfur in the test product is calculated by diluting the solution from the respective containersand culturing it on an agar plate (solidified gel base containing essential growth nutrients). The pathogen is then allowed to grow under appropriate lab conditions.

Step 3

Tracking The Growth Of Challenge Micro-organisms

Similar to the last step, the population count for the challenge microorganism is calculated by the cell plating method at different intervals of time.After each time duration, the concentration of the pathogen should reflect a steady decline as the test product works against them.

What's The Final Result?

For a product to have an anti-dandruff property, concentration of pathogen should decline as the test product works against the particular pathogen. At Areoveda, the tested products reveal similar results!